Quantifying EGFR endosomal recycling via immunofluorescence in breast cancer cells
Date
2022
Authors
Lonic, A.
Onglao, W.
Khew-Goodall, Y.
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Journal article
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STAR Protocols, 2022; 3(2):101305-1-101305-12
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Ana Lonic, Winona Onglao, and Yeesim Khew-Goodall
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Abstract
Previously published protocols for quantification of endosomal recycling are limited by the use of radioactive reagents, washing of cells in reducing buffers, or the requirement for large numbers of cells. Here, we describe a protocol for quantification of endosomal recycling using immunofluorescence that is optimized for EGFR in BT-549 breast cancer cells but could be applied to other RTKs and cell lines. Our protocol enables quick assessment of recycling and uses a relatively small number of cells.
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© 2022 The Authors. This article is available under the Creative Commons CC-BY-NC-ND license and permits non-commercial use of the work as published, without adaptation or alteration provided the work is fully attributed.