Quantifying EGFR endosomal recycling via immunofluorescence in breast cancer cells

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dc.contributor.authorLonic, A.
dc.contributor.authorOnglao, W.
dc.contributor.authorKhew-Goodall, Y.
dc.date.issued2022
dc.description.abstractPreviously published protocols for quantification of endosomal recycling are limited by the use of radioactive reagents, washing of cells in reducing buffers, or the requirement for large numbers of cells. Here, we describe a protocol for quantification of endosomal recycling using immunofluorescence that is optimized for EGFR in BT-549 breast cancer cells but could be applied to other RTKs and cell lines. Our protocol enables quick assessment of recycling and uses a relatively small number of cells.
dc.description.statementofresponsibilityAna Lonic, Winona Onglao, and Yeesim Khew-Goodall
dc.identifier.citationSTAR Protocols, 2022; 3(2):101305-1-101305-12
dc.identifier.doi10.1016/j.xpro.2022.101305
dc.identifier.issn2666-1667
dc.identifier.issn2666-1667
dc.identifier.orcidKhew-Goodall, Y. [0000-0002-0473-5392]
dc.identifier.urihttps://hdl.handle.net/2440/140358
dc.language.isoen
dc.publisherElsevier BV
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1146087
dc.rights© 2022 The Authors. This article is available under the Creative Commons CC-BY-NC-ND license and permits non-commercial use of the work as published, without adaptation or alteration provided the work is fully attributed.
dc.source.urihttps://doi.org/10.1016/j.xpro.2022.101305
dc.subjectCell Biology; Cancer; Microscopy; Signal Transduction
dc.subject.meshEndosomes
dc.subject.meshHumans
dc.subject.meshBreast Neoplasms
dc.subject.meshFluorescent Antibody Technique
dc.subject.meshStaining and Labeling
dc.subject.meshFemale
dc.subject.meshErbB Receptors
dc.titleQuantifying EGFR endosomal recycling via immunofluorescence in breast cancer cells
dc.typeJournal article
pubs.publication-statusPublished

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