A Method for in Vivo Quantification of Cytokine IL-1β in the Rat Intrathecal Space
Date
2020
Authors
Deng, F.
Arman, A.
Goldys, E.M.
Hutchinson, M.R.
Liu, G.
Editors
Advisors
Journal Title
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Type:
Journal article
Citation
ACS Applied Biomaterials, 2020; 3(1):539-546
Statement of Responsibility
Fei Deng, Azim Arman, Ewa M. Goldys, Mark R. Hutchinson, and Guozhen Liu
Conference Name
Abstract
IL-1β is a potent pro-inflammatory cytokine critical to multiple pathologies in the central nervous system (CNS). Quantification of IL-1β in vivo is challenging due to pM range of IL-1β released in the spinal cord and also the terminal nature of cerebrospinal fluid (CSF) sampling in rodents. Herein we developed a robust in vivo device on stainless steel suitable for detection of IL-1β in the spinal cord of rats. This approach offers high sensitivity (3.2 pg mL–1) and specificity to IL-1β. Also, a modified lumbar puncture method was employed to implant the device in the intrathecal space of male Sprague–Dawley (SD) rats under short-acting anesthesia, allowing minimal invasiveness, which provided the possibility of repeated measurement of IL-1β in the same animal. Our biosensing technology and the surgical method provide a universal platform for in vivo detection of diverse analytes in longitudinal, within-subject studies in the intrathecal space of rats to reduce the required number of experimental animals.
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Published online16 December 2019
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© 2019 American Chemical Society