A Method for in Vivo Quantification of Cytokine IL-1β in the Rat Intrathecal Space

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dc.contributor.authorDeng, F.
dc.contributor.authorArman, A.
dc.contributor.authorGoldys, E.M.
dc.contributor.authorHutchinson, M.R.
dc.contributor.authorLiu, G.
dc.date.issued2020
dc.descriptionPublished online16 December 2019
dc.description.abstractIL-1β is a potent pro-inflammatory cytokine critical to multiple pathologies in the central nervous system (CNS). Quantification of IL-1β in vivo is challenging due to pM range of IL-1β released in the spinal cord and also the terminal nature of cerebrospinal fluid (CSF) sampling in rodents. Herein we developed a robust in vivo device on stainless steel suitable for detection of IL-1β in the spinal cord of rats. This approach offers high sensitivity (3.2 pg mL–1) and specificity to IL-1β. Also, a modified lumbar puncture method was employed to implant the device in the intrathecal space of male Sprague–Dawley (SD) rats under short-acting anesthesia, allowing minimal invasiveness, which provided the possibility of repeated measurement of IL-1β in the same animal. Our biosensing technology and the surgical method provide a universal platform for in vivo detection of diverse analytes in longitudinal, within-subject studies in the intrathecal space of rats to reduce the required number of experimental animals.
dc.description.statementofresponsibilityFei Deng, Azim Arman, Ewa M. Goldys, Mark R. Hutchinson, and Guozhen Liu
dc.identifier.citationACS Applied Biomaterials, 2020; 3(1):539-546
dc.identifier.doi10.1021/acsabm.9b00958
dc.identifier.issn2576-6422
dc.identifier.issn2576-6422
dc.identifier.orcidHutchinson, M.R. [0000-0003-2154-5950]
dc.identifier.urihttps://hdl.handle.net/2440/139950
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.relation.granthttp://purl.org/au-research/grants/arc/FT160100039
dc.relation.granthttp://purl.org/au-research/grants/arc/FT180100565
dc.relation.granthttp://purl.org/au-research/grants/arc/CE140100003
dc.rights© 2019 American Chemical Society
dc.source.urihttps://doi.org/10.1021/acsabm.9b00958
dc.subjectspinal cord; cytokine sensing; IL-1β; rat intrathecal space; longitudinal experiments
dc.titleA Method for in Vivo Quantification of Cytokine IL-1β in the Rat Intrathecal Space
dc.title.alternativeA Method for in Vivo Quantification of Cytokine IL-1beta in the Rat Intrathecal Space
dc.typeJournal article
pubs.publication-statusPublished

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