A high-throughput plant DNA extraction method for marker analysis
Date
2003
Authors
Karakousis, A.
Langridge, P.
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Advisors
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Type:
Journal article
Citation
Plant Molecular Biology Reporter, 2003; 21(1):95a-95f
Statement of Responsibility
Angelo Karakousis and Peter Langridge
Conference Name
Abstract
The use of molecular markers to improve crops depends on the availability of rapid and efficient DNA extraction methods. Here we describe a simple and inexpensive method to isolate plant DNA suitable for RFLP, AFLP, and simple sequence repeat (SSR) analysis. This procedure uses stainless steel ball bearings to grind 16 samples simultaneously using a high-speed flask shaker. The method used in routine laboratory exercises yields 120–144 DNA extractions in a day by a single person at a cost of $0.60 (AUD) per sample, doubling the throughput of conventional methods.
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Dissertation Note
Provenance
Description
The original publication can be found at www.springerlink.com