A high-throughput plant DNA extraction method for marker analysis

dc.contributor.author	Karakousis, A.
dc.contributor.author	Langridge, P.
dc.date.issued	2003
dc.description	The original publication can be found at www.springerlink.com
dc.description.abstract	The use of molecular markers to improve crops depends on the availability of rapid and efficient DNA extraction methods. Here we describe a simple and inexpensive method to isolate plant DNA suitable for RFLP, AFLP, and simple sequence repeat (SSR) analysis. This procedure uses stainless steel ball bearings to grind 16 samples simultaneously using a high-speed flask shaker. The method used in routine laboratory exercises yields 120–144 DNA extractions in a day by a single person at a cost of $0.60 (AUD) per sample, doubling the throughput of conventional methods.
dc.description.statementofresponsibility	Angelo Karakousis and Peter Langridge
dc.identifier.citation	Plant Molecular Biology Reporter, 2003; 21(1):95a-95f
dc.identifier.doi	10.1007/BF02773402
dc.identifier.issn	0735-9640
dc.identifier.issn	1572-9818
dc.identifier.orcid	Langridge, P. [0000-0001-9494-400X]
dc.identifier.uri	http://hdl.handle.net/2440/40071
dc.language.iso	en
dc.publisher	Int Soc Plant Molecular Biology
dc.source.uri	http://www.springerlink.com/content/n11260nm5262h6w1/
dc.subject	AFLP
dc.subject	ball bearing
dc.subject	high throughput
dc.subject	plant DNA extraction
dc.subject	RFLP
dc.subject	SSR
dc.title	A high-throughput plant DNA extraction method for marker analysis
dc.type	Journal article
pubs.publication-status	Published

Collections