Intestinal absorption of fluorescently labeled nanoparticles
Date
2015
Authors
Simovic, S.
Song, Y.
Nann, T.
Desai, A.T.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Nanomedicine: Nanotechnology, Biology, and Medicine, 2015; 11(5):1169-1178
Statement of Responsibility
Conference Name
Abstract
Characterization of intestinal absorption of nanoparticles is critical in the design of noninvasive anticancer, protein-based, and genenanoparticle-based therapeutics. Here we demonstrate a general approach for the characterization of the intestinal absorption of nanoparticles and for understanding the mechanisms active in their processing within healthy intestinal cells. It is generally accepted that the cellular processing represents a major drawback of current nanoparticle-based therapeutic systems. In particular, endolysosomal trafficking causes degradation of therapeutic molecules such as proteins, lipids, acid-sensitive anticancer drugs, and genes. To date, investigations into nanoparticle processing within intestinal cells have studied mass transport through Caco-2 cells or everted rat intestinal sac models. We developed an approach to visualize directly the mechanisms of nanoparticle processing within intestinal tissue. These results clearly identify a mechanism by which healthy intestinal cells process nanoparticles and point to the possible use of this approach in the design of noninvasive nanoparticle-based therapies.
School/Discipline
Dissertation Note
Provenance
Description
Access Status
Rights
Copyright 2015 Elsevier
Access Condition Notes: Postprint only available on Open Access