Rapid bacterial evaluation beyond the colony forming unit in osteomyelitis
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(Published version)
Date
2024
Authors
Sun, Q.
Huynh, K.
Muratovic, D.
Gunn, N.J.
Zelmer, A.R.
Solomon, L.B.
Atkins, G.J.
Yang, D.
Editors
Kana, B.D.
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Journal article
Citation
eLife, 2024; 13:RP93698-1-RP93698-11
Statement of Responsibility
Qi Sun, Kimberley Huynh, Dzenita Muratovic, Nicholas J Gunn, Anja R Zelmer, Lucian Bogdan Solomon, Gerald J Atkins, Dongqing Yang
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Abstract
Examination of bacteria/host cell interactions is important for understanding the aetiology of many infectious diseases. The colony forming unit (CFU) has been the standard for quantifying bacterial burden for the past century, however, this suffers from low sensitivity and is dependent on bacterial culturability in vitro. Our data demonstrate the discrepancy between the CFU and bacterial genome copy number in an osteomyelitis-relevant co-culture system and we confirm diagnosis and quantify bacterial load in clinical bone specimens. This study provides an improved workflow for the quantification of bacterial burden in such cases.
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Version of Record published 24 June 2024
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© Copyright Sun et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.