A head impact model of early axonal injury in the sheep

Simple item page
dc.contributor.authorLewis, S.
dc.contributor.authorFinnie, J.
dc.contributor.authorBlumbergs, P.
dc.contributor.authorScott, G.
dc.contributor.authorManavis, J.
dc.contributor.authorBrown, C.
dc.contributor.authorReilly, P.
dc.contributor.authorJones, N.
dc.contributor.authorMcLean, J.
dc.date.issued1996
dc.description.abstractAxonal injury (AI), one of the principal determinants of clinical outcome after head injury, may evolve over several hours after injury, raising the future possibility of therapeutic intervention during this period. A new head impact model of AI in sheep was developed to examine pathological and physiological changes in the brain resulting from a graded traumatic insult. In this preliminary study 10 anesthetized and ventilated Merino ewes were used. Head injury was produced by impact from a humane stunner to the temporal region of an unrestrained head. Eight sheep were studied for 1, 2, 4, or 6 h after impact. Two sham animals (no impact, 6 h survival) were also examined. Arterial blood pressure, intracranial pressure, and cerebral blood flow were monitored continuously. A physiological index of injury severity was calculated by weighting the percentage shift from preinjury values for each monitored parameter over the first hour after injury. Immunostaining with amyloid precursor protein (APP) was used as a marker of axonal damage and the distribution of APP positive axons was recorded according to a sector scoring method (APPS). Widespread AI was identified in 7 of the 8 impacted animals, around cerebral contusions and in hemispheric white matter, central gray matter, brain stem, and cerebellum, and was detected as early as 1 h after injury. The degree of axonal injury (APPS) correlated well with an index of physiological response to injury (r = 0.83, p = 0.005).
dc.description.statementofresponsibilityStephen B. Lewis, John W. Finnie, Peter C. Blumbergs, Grace Scott, Jim Manavis, Chris Brown, Peter L. Reilly, Nigel R. Jones, and A.J. Mclean
dc.identifier.citationJournal of Neurotrauma, 1996; 13(9):505-514
dc.identifier.doi10.1089/neu.1996.13.505
dc.identifier.issn0897-7151
dc.identifier.issn1557-9042
dc.identifier.orcidFinnie, J. [0000-0003-2277-1693]
dc.identifier.orcidManavis, J. [0000-0001-7381-7781] [0000-0003-1268-561X]
dc.identifier.urihttp://hdl.handle.net/2440/17690
dc.language.isoen
dc.publisherMARY ANN LIEBERT INC PUBL
dc.rights© Mary Ann Liebert, Inc. publishers. All rights reserved, USA and worldwide.
dc.source.urihttps://doi.org/10.1089/neu.1996.13.505
dc.subjectamyloid precursor protein
dc.subjectaxonal injury
dc.subjecthead injury model
dc.subjectpathophysiological correlation
dc.subjectsheep
dc.titleA head impact model of early axonal injury in the sheep
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Centre for Automotive Safety Research publications