Fluorescence-activated cell sorting for analysis of cell type-specific responses to salinity stress in Arabidopsis and rice
Date
2012
Authors
Evrard, A.
Bargmann, B.
Birnbaum, K.
Tester, M.
Baumann, U.
Johnson, A.
Editors
Shabala, S.
Cuin, T.
Cuin, T.
Advisors
Journal Title
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Volume Title
Type:
Book chapter
Citation
Plant Salt Tolerance: Methods and Protocols, 2012 / Shabala, S., Cuin, T. (ed./s), vol.913, pp.265-276
Statement of Responsibility
Aurelie Evrard, Bastiaan O. R. Bargmann, Kenneth D. Birnbaum, Mark Tester, Ute Baumann, and Alexander A. T. Johnson
Conference Name
Abstract
Fluorescence-activated cell sorting (FACS) provides a rapid means of isolating large numbers of fluorescently tagged cells from a heterogeneous mixture of cells. Collections of transgenic plants with cell type-specific expression of fluorescent marker genes such as green fluorescent protein (GFP) are ideally suited for FACS-assisted studies of individual cell types. Here we describe the use of Arabidopsis and rice enhancer trap lines with tissue-specific GFP expression patterns in the root to isolate specific cell types of root tissues using FACS. Additionally, protocols are provided to impose a ramped salinity stress for 48 h prior to cell sorting.
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Dissertation Note
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© Springer Science+Business Media, LLC 2012