Anti-inflammatory activity of a lipid fraction (lyprinol) from the NZ green-lipped mussel
Date
1997
Authors
Whitehouse, M.
Macrides, T.
Kalafatis, N.
Betts, W.
Haynes, D.
Broadbent, J.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Inflammopharmacology: experimental and clinical studies, 1997; 5(3):237-246
Statement of Responsibility
Conference Name
Abstract
A lipid-rich extract, preparared by supercritical fluid extraction of fresh stabilized mussel powder (Lyprinol), showed significant anti- inflammatory (AI) activity given therapeutically and prophylactically po to Wistar and Dark Agouti rats developing either (a) adjuvant-induced polyarthritis or (b) collagen(II)-induced autoallergic arthritis, with ED<inf>50</inf>≤ 15 mg/kg; c.f. naproxen ≤ 25 mg/kg or various therapeutic oils (flaxseed, evening primrose, fish) ≤ 1800 mg/kg given orally. Lyprinol showed little or no activity in acute irritation assays (carrageenan, kaolin, histamine) indicating it is not mimicking rapid-acting NSAIDs. Incorporating Lyprinol into arthritigenic adjuvants composed of heat-killed Mycobacterium tuberculosis suspended in olive oil or squalane, effectively prevented arthritis development at a dose of 5 mg/rat. By contrast, 'dummy adjuvants' prepared with Mycobacterium tuberculosis and flaxseed, evening primrose or fish oils were still arthritigenic in Dark Agouti rats (doses of oil = 90 mg/rat). Lyprinol subfractions inhibited leukotriene-B<inf>4</inf> biosynthesis by stimulated human polymorphonuclear leukocytes in vitro, and prostaglandin- E<inf>2</inf> production by activated human macrophages in vitro. Much of this AI activity was associated with polyunsaturated fatty acids and natural antoxidants (carotenoids, etc.). In contrast to NSAIDs, Lyprinol is non- gastrotoxic in disease-stressed rats at 300 mg/kg po and does not seem to affect platelet aggregation (human, rat). These data show Lyprinol to be a reproducible, relatively stable, source of bioactive lipids with much greater potency than plant/marine oils currently used as nutritional supplements to ameliorate signs of inflammation.