Anti-inflammatory activity of a lipid fraction (lyprinol) from the NZ green-lipped mussel
| dc.contributor.author | Whitehouse, M. | |
| dc.contributor.author | Macrides, T. | |
| dc.contributor.author | Kalafatis, N. | |
| dc.contributor.author | Betts, W. | |
| dc.contributor.author | Haynes, D. | |
| dc.contributor.author | Broadbent, J. | |
| dc.date.issued | 1997 | |
| dc.description.abstract | A lipid-rich extract, preparared by supercritical fluid extraction of fresh stabilized mussel powder (Lyprinol), showed significant anti- inflammatory (AI) activity given therapeutically and prophylactically po to Wistar and Dark Agouti rats developing either (a) adjuvant-induced polyarthritis or (b) collagen(II)-induced autoallergic arthritis, with ED<inf>50</inf>≤ 15 mg/kg; c.f. naproxen ≤ 25 mg/kg or various therapeutic oils (flaxseed, evening primrose, fish) ≤ 1800 mg/kg given orally. Lyprinol showed little or no activity in acute irritation assays (carrageenan, kaolin, histamine) indicating it is not mimicking rapid-acting NSAIDs. Incorporating Lyprinol into arthritigenic adjuvants composed of heat-killed Mycobacterium tuberculosis suspended in olive oil or squalane, effectively prevented arthritis development at a dose of 5 mg/rat. By contrast, 'dummy adjuvants' prepared with Mycobacterium tuberculosis and flaxseed, evening primrose or fish oils were still arthritigenic in Dark Agouti rats (doses of oil = 90 mg/rat). Lyprinol subfractions inhibited leukotriene-B<inf>4</inf> biosynthesis by stimulated human polymorphonuclear leukocytes in vitro, and prostaglandin- E<inf>2</inf> production by activated human macrophages in vitro. Much of this AI activity was associated with polyunsaturated fatty acids and natural antoxidants (carotenoids, etc.). In contrast to NSAIDs, Lyprinol is non- gastrotoxic in disease-stressed rats at 300 mg/kg po and does not seem to affect platelet aggregation (human, rat). These data show Lyprinol to be a reproducible, relatively stable, source of bioactive lipids with much greater potency than plant/marine oils currently used as nutritional supplements to ameliorate signs of inflammation. | |
| dc.identifier.citation | Inflammopharmacology: experimental and clinical studies, 1997; 5(3):237-246 | |
| dc.identifier.doi | 10.1007/s10787-997-0002-0 | |
| dc.identifier.issn | 0925-4692 | |
| dc.identifier.issn | 1568-5608 | |
| dc.identifier.uri | http://hdl.handle.net/2440/5645 | |
| dc.language.iso | en | |
| dc.publisher | Springer Science and Business Media LLC | |
| dc.source.uri | https://doi.org/10.1007/s10787-997-0002-0 | |
| dc.title | Anti-inflammatory activity of a lipid fraction (lyprinol) from the NZ green-lipped mussel | |
| dc.type | Journal article | |
| pubs.publication-status | Published |