A combined free flow electrophoresis and DIGE approach to compare proteins in complex biological samples

Date

2012

Authors

Fung, K.Y.C.
Cursaro, C.
Lewanowitsch, T.
Cosgrove, L.
Hoffmann, P.

Editors

Kurien, B.J.
Scofield, R.H.

Advisors

Journal Title

Journal ISSN

Volume Title

Type:

Journal article

Citation

Methods in Molecular Biology, 2012; 869:135-146

Statement of Responsibility

Kim Y. C. Fung, Chris Cursaro, Tanya Lewanowitsch, Leah Cosgrove, Peter Hoffmann

Conference Name

DOI

10.1007/978-1-61779-821-4_12

Abstract

Free flow electrophoresis (FFE) has been applied in numerous studies as a protein separation technique due to its multiple advantages such as fast and efficient sample recovery, high resolving power, high reproducibility, and wide applicability to protein classes. As a stand-alone platform however, its utility in comparative proteomic analysis is limited as protein samples must be run sequentially rather than simultaneously which introduces inherent variability when attempting to perform quantitative analysis. Here we describe an approach combining fluorescent CyDye technology (DIGE) with FFE to simultaneously separate and identify differentially expressed proteins in a model cell system.

School/Discipline

Dissertation Note

Provenance

Description

Access Status

Rights

© Springer Science+Business Media B.V. 2012

License

Grant ID

Published Version

https://doi.org/10.1007/978-1-61779-821-4_12

Call number

Persistent link to this record

http://hdl.handle.net/2440/91119