A combined free flow electrophoresis and DIGE approach to compare proteins in complex biological samples

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dc.contributor.authorFung, K.Y.C.
dc.contributor.authorCursaro, C.
dc.contributor.authorLewanowitsch, T.
dc.contributor.authorCosgrove, L.
dc.contributor.authorHoffmann, P.
dc.contributor.editorKurien, B.J.
dc.contributor.editorScofield, R.H.
dc.date.issued2012
dc.description.abstractFree flow electrophoresis (FFE) has been applied in numerous studies as a protein separation technique due to its multiple advantages such as fast and efficient sample recovery, high resolving power, high reproducibility, and wide applicability to protein classes. As a stand-alone platform however, its utility in comparative proteomic analysis is limited as protein samples must be run sequentially rather than simultaneously which introduces inherent variability when attempting to perform quantitative analysis. Here we describe an approach combining fluorescent CyDye technology (DIGE) with FFE to simultaneously separate and identify differentially expressed proteins in a model cell system.
dc.description.statementofresponsibilityKim Y. C. Fung, Chris Cursaro, Tanya Lewanowitsch, Leah Cosgrove, Peter Hoffmann
dc.identifier.citationMethods in Molecular Biology, 2012; 869:135-146
dc.identifier.doi10.1007/978-1-61779-821-4_12
dc.identifier.isbn9781617798214
dc.identifier.issn1064-3745
dc.identifier.issn1940-6029
dc.identifier.urihttp://hdl.handle.net/2440/91119
dc.language.isoen
dc.publisherHumana Press
dc.relation.ispartofseriesMethods in Molecular Biology; 869
dc.rights© Springer Science+Business Media B.V. 2012
dc.source.urihttps://doi.org/10.1007/978-1-61779-821-4_12
dc.subjectFree flow electrophoresis
dc.subjectFFE
dc.subjectDIGE
dc.subjectProteomics
dc.titleA combined free flow electrophoresis and DIGE approach to compare proteins in complex biological samples
dc.typeJournal article
pubs.publication-statusPublished

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