Multispectral autofluorescence characteristics of reproductive aging in old and young mouse oocytes

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Date

2022

Authors

Campbell, J.M.
Mahbub, S.B.
Bertoldo, M.J.
Habibalahi, A.
Goss, D.M.
Ledger, W.L.
Gilchrist, R.B.
Wu, L.E.
Goldys, E.M.

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Journal Title

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Journal article

Citation

Biogerontology, 2022; 23(2):237-249

Statement of Responsibility

Jared M. Campbell, Saabah B. Mahbub, Michael J. Bertoldo, Abbas Habibalahi, Dale M. Goss, William L. Ledger, Robert B. Gilchrist, Lindsay E. Wu, Ewa M. Goldys

Conference Name

DOI

10.1007/s10522-022-09957-y

Abstract

Increasing age has a major detrimental impact on female fertility, which, with an ageing population, has major sociological implications. This impact is primarily mediated through deteriorating quality of the oocyte. Deteriorating oocyte quality with biological age is the greatest rate-limiting factor to female fertility. Here we have used label-free, non-invasive multi-spectral imaging to identify unique autofluorescence profiles of oocytes from young and aged animals. Discriminant analysis demonstrated that young oocytes have a distinct autofluorescent profile which accurately distinguishes them from aged oocytes. We recently showed that treatment with the nicotinamide adenine dinucleotide (NAD+) precursor nicotinamide mononucleotide (NMN) restored oocyte quality and fertility in aged animals, and when our analysis was applied to oocytes from aged animals treated with NMN, 85% of these oocytes were classified as having the autofluorescent signature of young animals. Spectral unmixing using the Robust Dependent Component Analysis (RoDECA) algorithm demonstrated that NMN treatment altered the metabolic profile of oocytes, increasing free NAD(P)H, protein bound NAD(P)H, redox ratio and the ratio of bound to free NAD(P)H. The frequency of oocytes with simultaneously high NAD(P)H and flavin content was also significantly increased in mice treated with NMN. Young and Aged + NMN oocytes had a smoother spectral distribution, with the distribution of NAD(P)H in young oocytes specifically differing from that of aged oocytes. Identifying the multispectral profile of oocyte autofluorescence during aging could have utility as a non-invasive and sensitive measure of oocyte quality.

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Published online: 24 February 2022

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© The Author(s) 2022. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Grant ID

http://purl.org/au-research/grants/nhmrc/1103689
 http://purl.org/au-research/grants/nhmrc/1122484
 http://purl.org/au-research/grants/nhmrc/1139763
 http://purl.org/au-research/grants/nhmrc/1044295
 http://purl.org/au-research/grants/nhmrc/1066172
 http://purl.org/au-research/grants/nhmrc/1127821
 http://purl.org/au-research/grants/arc/DP170101863
 http://purl.org/au-research/grants/arc/DP210102960
 http://purl.org/au-research/grants/arc/CE140100003

Published Version

https://doi.org/10.1007/s10522-022-09957-y

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Persistent link to this record

https://hdl.handle.net/2440/134480