Towards multimodal cellular imaging: optical and X-ray fluorescence

Date

2023

Authors

Graziotto, M.E.
Kidman, C.J.
Adair, L.D.
James, S.A.
Harris, H.H.
New, E.J.

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Journal article

Citation

Chemical Society Reviews, 2023; 52(23):8295-8318

Statement of Responsibility

Marcus E. Graziotto, Clinton J. Kidman, Liam D. Adair, Simon A. James, Hugh H. Harris and Elizabeth J. New

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Abstract

Imaging techniques permit the study of the molecular interactions that underlie health and disease. Each imaging technique collects unique chemical information about the cellular environment. Multimodal imaging, using a single probe that can be detected by multiple imaging modalities, can maximise the information extracted from a single cellular sample by combining the results of different imaging techniques. Of particular interest in biological imaging is the combination of the specificity and sensitivity of optical fluorescence microscopy (OFM) with the quantitative and element-specific nature of X-ray fluorescence microscopy (XFM). Together, these techniques give a greater understanding of how native elements or therapeutics affect the cellular environment. This review focuses on recent studies where both techniques were used in conjunction to study cellular systems, demonstrating the breadth of biological models to which this combination of techniques can be applied and the potential for these techniques to unlock untapped knowledge of disease states.

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Dissertation Note

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First published 01 Nov 2023

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© The Royal Society of Chemistry 2023

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