LRGUK-1 is required for basal body and manchette function during spermatogenesis and male fertility

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dc.contributor.authorLiu, Y.
dc.contributor.authorDeBoer, K.
dc.contributor.authorde Kretser, D.
dc.contributor.authorO Donnell, L.
dc.contributor.authorO Connor, A.
dc.contributor.authorMerriner, D.
dc.contributor.authorOkuda, H.
dc.contributor.authorWhittle, B.
dc.contributor.authorJans, D.
dc.contributor.authorEfthymiadis, A.
dc.contributor.authorMcLachlan, R.
dc.contributor.authorOrmandy, C.
dc.contributor.authorGoodnow, C.
dc.contributor.authorJamsai, D.
dc.contributor.authorO Bryan, M.
dc.contributor.editorYan, W.
dc.date.issued2015
dc.description.abstractMale infertility affects at least 5% of reproductive age males. The most common pathology is a complex presentation of decreased sperm output and abnormal sperm shape and motility referred to as oligoasthenoteratospermia (OAT). For the majority of OAT men a precise diagnosis cannot be provided. Here we demonstrate that leucine-rich repeats and guanylate kinase-domain containing isoform 1 (LRGUK-1) is required for multiple aspects of sperm assembly, including acrosome attachment, sperm head shaping and the initiation of the axoneme growth to form the core of the sperm tail. Specifically, LRGUK-1 is required for basal body attachment to the plasma membrane, the appropriate formation of the sub-distal appendages, the extension of axoneme microtubules and for microtubule movement and organisation within the manchette. Manchette dysfunction leads to abnormal sperm head shaping. Several of these functions may be achieved in association with the LRGUK-1 binding partner HOOK2. Collectively, these data establish LRGUK-1 as a major determinant of microtubule structure within the male germ line.
dc.description.statementofresponsibilityYan Liu, Kathleen DeBoer, David M. de Kretser, Liza O, Donnell, Anne E. O, Connor, D. Jo Merriner, Hidenobu Okuda, Belinda Whittle, David A. Jans, Athina Efthymiadis, Robert I. McLachlan, Christopher J. Ormandy, Chris C. Goodnow, Duangporn Jamsai, Moira K. O, Bryan
dc.identifier.citationPLoS Genetics, 2015; 11(3):1-20
dc.identifier.doi10.1371/journal.pgen.1005090
dc.identifier.issn1553-7390
dc.identifier.issn1553-7404
dc.identifier.urihttp://hdl.handle.net/2440/97197
dc.language.isoen
dc.publisherPublic Library of Science
dc.relation.grantNHMRC
dc.relation.grantARC
dc.rights© 2015 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
dc.source.urihttps://doi.org/10.1371/journal.pgen.1005090
dc.subjectTestis
dc.subjectSpermatozoa
dc.subjectCell Membrane
dc.subjectAnimals
dc.subjectMice, Inbred C57BL
dc.subjectHumans
dc.subjectMice
dc.subjectInfertility, Male
dc.subjectMicrotubule-Associated Proteins
dc.subjectProtein Isoforms
dc.subjectSequence Alignment
dc.subjectSpermatogenesis
dc.subjectAmino Acid Sequence
dc.subjectMolecular Sequence Data
dc.subjectMale
dc.subjectBasal Bodies
dc.subjectGuanylate Kinases
dc.titleLRGUK-1 is required for basal body and manchette function during spermatogenesis and male fertility
dc.typeJournal article
pubs.publication-statusPublished

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