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Chromosomes distribute randomly to, but not within, human neutrophil nuclear lobes

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hdl_149445.pdf (5.61 MB)
  (Published version)

Date

2021

Authors

Keenan, C.R.
Mlodzianoski, M.J.
Coughlan, H.D.
Bediaga, N.G.
Naselli, G.
Lucas, E.C.
Wang, Q.
de Graaf, C.A.
Hilton, D.J.
Harrison, L.C.

Editors

Advisors

Journal Title

Journal ISSN

Volume Title

Type:

Journal article

Citation

iScience, 2021; 24(3):102161-1-102161-24

Statement of Responsibility

Christine R. Keenan, Michael J. Mlodzianoski, Hannah D. Coughlan, Naiara G. Bediaga, Gaetano Naselli, Erin C. Lucas, Qike Wang, Carolyn A. de Graaf, Douglas J. Hilton, Leonard C. Harrison, Gordon K. Smyth, Kelly L. Rogers, Thomas Boudier, Rhys S. Allan, Timothy M. Johanson

Conference Name

DOI

10.1016/j.isci.2021.102161

Abstract

The proximity pattern and radial distribution of chromosome territories within spherical nuclei are random and non-random, respectively. Whether this distribution pattern is conserved in the partitioned or lobed nuclei of polymorphonuclear cells is unclear. Here we use chromosome paint technology to examine the chromosome territories of all 46 chromosomes in hundreds of single human neutrophils – an abundant and famously polymorphonuclear immune cell. By comparing the distribution of chromosomes to randomly shuffled controls and validating with orthogonal chromosome conformation capture technology, we show for the first time that human chromosomes randomly distribute to neutrophil nuclear lobes, while maintaining a non-random radial distribution within these lobes. Furthermore, we demonstrate that chromosome length correlates with three-dimensional volume not only in neutrophils but other human immune cells. This work demonstrates that chromosomes are largely passive passengers during the neutrophil lobing process but are able to subsequently maintain their macro-level organization within lobes.

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Dissertation Note

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Description

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Rights

© 2021 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Grant ID

http://purl.org/au-research/grants/nhmrc/1124081
 http://purl.org/au-research/grants/nhmrc/1049307
 http://purl.org/au-research/grants/nhmrc/1100451
 http://purl.org/au-research/grants/nhmrc/1058892
 http://purl.org/au-research/grants/nhmrc/1158531
 http://purl.org/au-research/grants/nhmrc/1125436
 http://purl.org/au-research/grants/nhmrc/1113577
 http://purl.org/au-research/grants/nhmrc/1080887
 http://purl.org/au-research/grants/nhmrc/1037321

Published Version

https://doi.org/10.1016/j.isci.2021.102161

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Persistent link to this record

https://hdl.handle.net/2440/149445