Kinetics of human immunodeficiency virus type 1 (HIV) DNA integration in acutely infected cells as determined using a novel assay for detection of integrated HIV DNA
Date
2001
Authors
Vandegraaff, N.
Kumar, R.
Burrell, C.
Li, P.
Editors
Advisors
Journal Title
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Volume Title
Type:
Journal article
Citation
Journal of Virology, 2001; 75(22):11253-11260
Statement of Responsibility
Nick Vandegraaff, Raman Kumar, Christopher J. Burrell, and Peng Li
Conference Name
Abstract
We have developed a novel linker-primer PCR assay for the detection and quantification of integrated human immunodeficiency virus type 1 (HIV) DNA. This assay reproducibly allowed the detection of 10 copies of integrated HIV DNA, in a background of 2 × 105 cell equivalents of human chromosomal DNA, without amplifying extrachromosomal HIV DNA. We have used this assay and a near-synchronous one-step T-cell infection model to investigate the kinetics of viral DNA accumulation following HIV infection. We report here that integrated HIV DNA started accumulating 1 h after the first appearance of extrachromosomal viral DNA and accounted for ~10% of the total HIV DNA synthesized in the first round of viral replication. These results highlight the efficient nature of integrase-mediated HIV integration in infected T cells.
School/Discipline
Dissertation Note
Provenance
Description
Copyright © 2001, American Society for Microbiology