Kinetics of human immunodeficiency virus type 1 (HIV) DNA integration in acutely infected cells as determined using a novel assay for detection of integrated HIV DNA

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dc.contributor.authorVandegraaff, N.
dc.contributor.authorKumar, R.
dc.contributor.authorBurrell, C.
dc.contributor.authorLi, P.
dc.date.issued2001
dc.descriptionCopyright © 2001, American Society for Microbiology
dc.description.abstractWe have developed a novel linker-primer PCR assay for the detection and quantification of integrated human immunodeficiency virus type 1 (HIV) DNA. This assay reproducibly allowed the detection of 10 copies of integrated HIV DNA, in a background of 2 × 105 cell equivalents of human chromosomal DNA, without amplifying extrachromosomal HIV DNA. We have used this assay and a near-synchronous one-step T-cell infection model to investigate the kinetics of viral DNA accumulation following HIV infection. We report here that integrated HIV DNA started accumulating 1 h after the first appearance of extrachromosomal viral DNA and accounted for ~10% of the total HIV DNA synthesized in the first round of viral replication. These results highlight the efficient nature of integrase-mediated HIV integration in infected T cells.
dc.description.statementofresponsibilityNick Vandegraaff, Raman Kumar, Christopher J. Burrell, and Peng Li
dc.identifier.citationJournal of Virology, 2001; 75(22):11253-11260
dc.identifier.doi10.1128/JVI.75.22.11253-11260.2001
dc.identifier.issn0022-538X
dc.identifier.issn1098-5514
dc.identifier.orcidBurrell, C. [0000-0002-4020-349X]
dc.identifier.urihttp://hdl.handle.net/2440/28092
dc.language.isoen
dc.publisherAmer Soc Microbiology
dc.source.urihttp://jvi.asm.org/cgi/content/abstract/75/22/11253
dc.subjectT-Lymphocytes
dc.subjectCell Line
dc.subjectHumans
dc.subjectHIV-1
dc.subjectDNA, Viral
dc.subjectPolymerase Chain Reaction
dc.subjectVirus Integration
dc.subjectVirus Replication
dc.subjectKinetics
dc.titleKinetics of human immunodeficiency virus type 1 (HIV) DNA integration in acutely infected cells as determined using a novel assay for detection of integrated HIV DNA
dc.typeJournal article
pubs.publication-statusPublished

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