Foxp3 heterozygosity does not overtly affect mammary gland development during puberty or the oestrous cycle in mice

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dc.contributor.authorAtashgaran, V.
dc.contributor.authorDasari, P.
dc.contributor.authorHodson, L.J.
dc.contributor.authorEvdokiou, A.
dc.contributor.authorBarry, S.C.
dc.contributor.authorIngman, W.V.
dc.date.issued2020
dc.description.abstractFemale mice heterozygous for a genetic mutation in transcription factor forkhead box p3 (Foxp3) spontaneously develop mammary cancers; however, the underlying mechanism is not well understood. We hypothesised that increased cancer susceptibility is associated with an underlying perturbation in mammary gland development. The role of Foxp3 in mammary ductal morphogenesis was investigated in heterozygous Foxp3Sf+ and wildtype Foxp3 +/+ mice during puberty and at specific stages of the oestrous cycle. No differences in mammary ductal branching morphogenesis, terminal end bud formation or ductal elongation were observed in pubertal Foxp3Sf/+ mice compared with Foxp3 +/+ mice. During adulthood, all mice underwent normal regular oestrous cycles. No differences in epithelial branching morphology were detected in mammary glands from mice at the oestrus, metoestrus, dioestrus and pro-oestrus stages of the cycle. Furthermore, abundance of Foxp3 mRNA and protein in the mammary gland and lymph nodes was not altered in Foxp3Sf/+ mice compared with Foxp3 +/+ mice. These studies suggest that Foxp3 heterozygosity does not overtly affect mammary gland development during puberty or the oestrous cycle. Further studies are required to dissect the underlying mechanisms of increased mammary cancer susceptibility in Foxp3Sf/+ heterozygous mice and the function of this transcription factor in normal mammary gland development.
dc.description.statementofresponsibilityVahid Atashgaran, Pallave Dasari, Leigh J. Hodson, Andreas Evdokiou, Simon C. Barry, and Wendy V. Ingman
dc.identifier.citationReproduction, Fertility and Development, 2020; 32(8):774-782
dc.identifier.doi10.1071/RD19378
dc.identifier.issn1031-3613
dc.identifier.issn1448-5990
dc.identifier.orcidDasari, P. [0000-0001-8399-1740]
dc.identifier.orcidEvdokiou, A. [0000-0001-8321-9806]
dc.identifier.orcidBarry, S.C. [0000-0002-0597-7609]
dc.identifier.orcidIngman, W.V. [0000-0003-3116-2902]
dc.identifier.urihttps://hdl.handle.net/2440/146236
dc.language.isoen
dc.publisherCSIRO Publishing
dc.rights© CSIRO 2020
dc.source.urihttps://doi.org/10.1071/rd19378
dc.subjectdevelopmental biology; ductal epithelium; mammary gland morphogenesis; mouse model; regulatory T cells; terminal end bud.
dc.subject.meshMammary Glands, Animal
dc.subject.meshLymph Nodes
dc.subject.meshAnimals
dc.subject.meshMice, Inbred C57BL
dc.subject.meshMice
dc.subject.meshMammary Neoplasms, Animal
dc.subject.meshRNA, Messenger
dc.subject.meshMorphogenesis
dc.subject.meshEstrous Cycle
dc.subject.meshSexual Maturation
dc.subject.meshHeterozygote
dc.subject.meshMutation
dc.subject.meshFemale
dc.subject.meshForkhead Transcription Factors
dc.titleFoxp3 heterozygosity does not overtly affect mammary gland development during puberty or the oestrous cycle in mice
dc.typeJournal article
pubs.publication-statusPublished

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