A genome-wide transcriptomic analysis of embryos fathered by obese males in a murine model of diet-induced obesity

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dc.contributor.authorBernhardt, L.
dc.contributor.authorDittrich, M.
dc.contributor.authorEl-Merahbi, R.
dc.contributor.authorSaliba, A.E.
dc.contributor.authorMüller, T.
dc.contributor.authorSumara, G.
dc.contributor.authorVogel, J.
dc.contributor.authorNichols-Burns, S.
dc.contributor.authorMitchell, M.
dc.contributor.authorHaaf, T.
dc.contributor.authorEl Hajj, N.
dc.date.issued2021
dc.description.abstractPaternal obesity is known to have a negative impact on the male’s reproductive health as well as the health of his offspring. Although epigenetic mechanisms have been implicated in the nongenetic transmission of acquired traits, the effect of paternal obesity on gene expression in the preimplantation embryo has not been fully studied. To this end, we investigated whether paternal obesity is associated with gene expression changes in eight-cell stage embryos fathered by males on a high-fat diet. We used single embryo RNA-seq to compare the gene expression profile of embryos generated by males on a high fat (HFD) versus control (CD) diet. This analysis revealed significant upregulation of the Samd4b and Gata6 gene in embryos in response to a paternal HFD. Furthermore, we could show a significant increase in expression of both Gata6 and Samd4b during differentiation of stromal vascular cells into mature adipocytes. These findings suggest that paternal obesity may induce changes in the male germ cells which are associated with the gene expression changes in the resulting preimplantation embryos.
dc.description.statementofresponsibilityLaura Bernhardt, Marcus Dittrich, Rabih El-Merahbi, Antoine-Emmanuel Saliba, Tobias Müller, Grzegorz Sumara, Jörg Vogel, Stefanie Nichols-Burns, Megan Mitchell, Thomas Haaf, Nady El Hajj
dc.identifier.citationScientific Reports, 2021; 11(1):1979-1-1979-7
dc.identifier.doi10.1038/s41598-021-81226-3
dc.identifier.issn2045-2322
dc.identifier.issn2045-2322
dc.identifier.orcidMitchell, M. [0000-0002-4284-2201]
dc.identifier.urihttps://hdl.handle.net/2440/145750
dc.language.isoen
dc.publisherSpringer Science and Business Media LLC
dc.rights© The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
dc.source.urihttps://doi.org/10.1038/s41598-021-81226-3
dc.subjectDevelopment; Epigenetic memory; Gene expression; Gene regulation; Obesity
dc.subject.meshBlastocyst
dc.subject.meshAnimals
dc.subject.meshMice, Inbred C57BL
dc.subject.meshHumans
dc.subject.meshMice
dc.subject.meshObesity
dc.subject.meshDisease Models, Animal
dc.subject.meshRepressor Proteins
dc.subject.meshFathers
dc.subject.meshGene Expression Regulation
dc.subject.meshEpigenesis, Genetic
dc.subject.meshEmbryonic Development
dc.subject.meshGenome, Human
dc.subject.meshMale
dc.subject.meshGATA6 Transcription Factor
dc.subject.meshEmbryo, Mammalian
dc.subject.meshTranscriptome
dc.subject.meshDiet, High-Fat
dc.subject.meshRNA-Seq
dc.titleA genome-wide transcriptomic analysis of embryos fathered by obese males in a murine model of diet-induced obesity
dc.typeJournal article
pubs.publication-statusPublished

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