Axonal transport of BDNF precursor in primary sensory neurons
Date
2006
Authors
Wang, H.
Linyan Wu, L.
Song, X.
Luo, X.
Zhong, J.
Rush, R.
Zhou, X.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
European Journal of Neuroscience, 2006; 24(9):2444-2452
Statement of Responsibility
Hui Wang, Linda Lin-yan Wu, Xing-Yun Song, Xue-Gang Luo, Jin-Hua Zhong, Robert A. Rush and Xin-Fu Zhou
Conference Name
Abstract
Recent studies have shown that the precursor of brain-derived neurotrophic factor (pro-BDNF) activates p75NTR with high affinity to induce apoptosis. Here we show that pro-BDNF is transported anterogradely and retrogradely in sensory neurons of adult rats. After a crush injury of sciatic nerves, dorsal roots or dorsal column in adult Sprague–Dawley rats, the immunoreactivity for pro-BDNF accumulated at both the proximal and distal segments. The accumulation reached a maximum at 24 h after injury. Western blot analysis also revealed pro-BDNF in sciatic nerve segments proximal and distal to the ligature and in the spinal cord. Biotinylated or Alexa-488-labelled pro-BDNF injected into sciatic nerve was internalized and transported both retrogradely and anterogradely within sensory neurons. These results demonstrate that pro-BDNF is anterogradely and retrogradely transported in sensory neurons, suggesting that endogenous pro-BDNF may be released and play important functions.