Axonal transport of BDNF precursor in primary sensory neurons

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dc.contributor.authorWang, H.
dc.contributor.authorLinyan Wu, L.
dc.contributor.authorSong, X.
dc.contributor.authorLuo, X.
dc.contributor.authorZhong, J.
dc.contributor.authorRush, R.
dc.contributor.authorZhou, X.
dc.date.issued2006
dc.description.abstractRecent studies have shown that the precursor of brain-derived neurotrophic factor (pro-BDNF) activates p75NTR with high affinity to induce apoptosis. Here we show that pro-BDNF is transported anterogradely and retrogradely in sensory neurons of adult rats. After a crush injury of sciatic nerves, dorsal roots or dorsal column in adult Sprague–Dawley rats, the immunoreactivity for pro-BDNF accumulated at both the proximal and distal segments. The accumulation reached a maximum at 24 h after injury. Western blot analysis also revealed pro-BDNF in sciatic nerve segments proximal and distal to the ligature and in the spinal cord. Biotinylated or Alexa-488-labelled pro-BDNF injected into sciatic nerve was internalized and transported both retrogradely and anterogradely within sensory neurons. These results demonstrate that pro-BDNF is anterogradely and retrogradely transported in sensory neurons, suggesting that endogenous pro-BDNF may be released and play important functions.
dc.description.statementofresponsibilityHui Wang, Linda Lin-yan Wu, Xing-Yun Song, Xue-Gang Luo, Jin-Hua Zhong, Robert A. Rush and Xin-Fu Zhou
dc.identifier.citationEuropean Journal of Neuroscience, 2006; 24(9):2444-2452
dc.identifier.doi10.1111/j.1460-9568.2006.05138.x
dc.identifier.issn0953-816X
dc.identifier.issn1460-9568
dc.identifier.orcidZhou, X. [0000-0002-8687-0175]
dc.identifier.urihttp://hdl.handle.net/2440/55258
dc.language.isoen
dc.publisherBlackwell Science Ltd
dc.source.urihttps://doi.org/10.1111/j.1460-9568.2006.05138.x
dc.subjectSpinal Cord
dc.subjectNeurons, Afferent
dc.subjectSciatic Nerve
dc.subjectSpinal Nerve Roots
dc.subjectAnimals
dc.subjectMice
dc.subjectRats
dc.subjectRats, Sprague-Dawley
dc.subjectBrain-Derived Neurotrophic Factor
dc.subjectBlotting, Western
dc.subjectNerve Crush
dc.subjectAxonal Transport
dc.titleAxonal transport of BDNF precursor in primary sensory neurons
dc.typeJournal article
pubs.publication-statusPublished

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