25-Hydroxyvitamin D requirement for maintaining skeletal health utilising a Sprague-Dawley rat model
Date
2007
Authors
Anderson, P.
Sawyer, A.
May, B.
O'Loughlin, P.
Morris, H.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
The Journal of Steroid Biochemistry and Molecular Biology, 2007; 103(3-5 Sp Iss):592-595
Statement of Responsibility
P.H. Anderson, R.K. Sawyer, B.K. May, P.D. O’Loughlin and H.A. Morris
Conference Name
Abstract
To study the role of vitamin D to optimise bone architecture, we have developed an animal model to investigate the effects of frank vitamin D-deficiency as well as graded depletion of circulating 25-hydroxyvitamin D₃ (25D) levels on the skeleton. Rats fed on dietary vitamin D levels from 0 to 500 ng/day achieved diet-dependent circulating levels of 25D ranging from 11 to 115 nmol/L. Levels of serum 1,25-dihydroxyvitamin D₃ (1,25D) increased as dietary vitamin D increased between 0 and 200 ng/day at which point a maximum level was achieved and retained with higher vitamin D intakes. The renal levels of 25-hydroxyvitamin D-1α-hydroxylase (CYP27B1) mRNA were highest in animal groups fed on vitamin D between 0 and 300 ng/day. In contrast, renal 25-hydroxyvitamin D 24-hydroxylase (CYP24) mRNA levels increased as dietary vitamin D increased achieving maximum levels in animals receiving 500 ng vitamin D/day. This animal model of vitamin D depletion is suitable to provide invaluable information on the serum levels of 25D and dietary calcium intake necessary for optimal bone structure. Such information is essential for developing nutritional recommendations to reduce the incidence of osteoporotic hip fractures.
School/Discipline
Dissertation Note
Provenance
Description
Special Issue: Proceedings of the 13th Workshop on Vitamin D (Victoria, British Columbia, Canada, April 2006). Edited by R. Bouillon, A.W. Norman and J.R. Pasqualini Copyright © 2007 Elsevier Ltd All rights reserved.