25-Hydroxyvitamin D requirement for maintaining skeletal health utilising a Sprague-Dawley rat model

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dc.contributor.authorAnderson, P.
dc.contributor.authorSawyer, A.
dc.contributor.authorMay, B.
dc.contributor.authorO'Loughlin, P.
dc.contributor.authorMorris, H.
dc.date.issued2007
dc.descriptionSpecial Issue: Proceedings of the 13th Workshop on Vitamin D (Victoria, British Columbia, Canada, April 2006). Edited by R. Bouillon, A.W. Norman and J.R. Pasqualini Copyright © 2007 Elsevier Ltd All rights reserved.
dc.description.abstractTo study the role of vitamin D to optimise bone architecture, we have developed an animal model to investigate the effects of frank vitamin D-deficiency as well as graded depletion of circulating 25-hydroxyvitamin D₃ (25D) levels on the skeleton. Rats fed on dietary vitamin D levels from 0 to 500 ng/day achieved diet-dependent circulating levels of 25D ranging from 11 to 115 nmol/L. Levels of serum 1,25-dihydroxyvitamin D₃ (1,25D) increased as dietary vitamin D increased between 0 and 200 ng/day at which point a maximum level was achieved and retained with higher vitamin D intakes. The renal levels of 25-hydroxyvitamin D-1α-hydroxylase (CYP27B1) mRNA were highest in animal groups fed on vitamin D between 0 and 300 ng/day. In contrast, renal 25-hydroxyvitamin D 24-hydroxylase (CYP24) mRNA levels increased as dietary vitamin D increased achieving maximum levels in animals receiving 500 ng vitamin D/day. This animal model of vitamin D depletion is suitable to provide invaluable information on the serum levels of 25D and dietary calcium intake necessary for optimal bone structure. Such information is essential for developing nutritional recommendations to reduce the incidence of osteoporotic hip fractures.
dc.description.statementofresponsibilityP.H. Anderson, R.K. Sawyer, B.K. May, P.D. O’Loughlin and H.A. Morris
dc.description.urihttp://www.elsevier.com/wps/find/journaldescription.cws_home/333/description#description
dc.identifier.citationThe Journal of Steroid Biochemistry and Molecular Biology, 2007; 103(3-5 Sp Iss):592-595
dc.identifier.doi10.1016/j.jsbmb.2006.12.086
dc.identifier.issn0960-0760
dc.identifier.issn1879-1220
dc.identifier.orcidAnderson, P. [0000-0002-8685-3252]
dc.identifier.orcidMorris, H. [0000-0002-2745-3750]
dc.identifier.urihttp://hdl.handle.net/2440/39955
dc.language.isoen
dc.publisherPergamon-Elsevier Science Ltd
dc.source.urihttps://doi.org/10.1016/j.jsbmb.2006.12.086
dc.subjectVitamin D-deficiency
dc.subjectKidney
dc.subjectOsteomalacia
dc.subjectOsteoporosis
dc.subject25-Hydroxyvitamin D-1α-hydroxylase
dc.subject25-Hydroxyvitamin D-24 hydroxylase
dc.title25-Hydroxyvitamin D requirement for maintaining skeletal health utilising a Sprague-Dawley rat model
dc.typeJournal article
pubs.publication-statusPublished

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