Molecular characterization and pathogenicity evaluation of enterovirus G isolated from diarrheic piglets
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(Published version)
Date
2023
Authors
Ibrahim, Y.M.
Zhang, W.
Wang, X.
Werid, G.M.
Fu, L.
Yu, H.
Wang, Y.
Editors
He, B.
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Journal article
Citation
Microbiology Spectrum, 2023; 11(6)
Statement of Responsibility
Yassein M. Ibrahim, Wenli Zhang, Xinrong Wang, Gebremeskel Mamu Werid, Lizhi Fu, Haidong Yu, Yue Wang
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Abstract
Four enterovirus G (EV-G) strains were isolated from diarrheic piglets that were negative for common swine enteric viruses. The spherical enterovirus particles of roughly 30-nm diameter were observed under transmission electron microscopy by using plaque-purified enterovirus. The complete genome sequence analysis revealed that each of four enteroviruses contained a papain-like cysteine protease (PLCP) gene between the 2C and 3A junction regions of the viral genome. This insertion encoded a predicted protease similar to the PLCP of porcine torovirus. The phylogenetic analysis based on complete genome with and without PLCP gene revealed that the four isolated EV-G strains were grouped together with global enterovirus G1-PLCP strains, and more closely related to EV-G/PLCP strains previously detected in China, Japan, and Korea (90.3%–92.2% similarities based on nucleotides). The cell susceptibility test demonstrated that the isolated EV-G could infect and replicate in cell lines from various host species. Furthermore, pathogenicity evaluation showed that the isolated EV-Gs induced mild diarrhea, pyrexia, and reduced body weight in infected piglets. The epidemiological investigation revealed a high prevalence of EV-G in swine herds. Together, our findings demonstrate that the isolated EV-G is pathogenic in piglets and may be advantageous in providing more trustworthy data on the evolution and pathological properties of EV-G.
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Dissertation Note
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Published 13 October 2023
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Copyright © 2023 Ibrahim et al. This is an openaccess article distributed under the terms of the Creative Commons Attribution 4.0 International license.