Molecular characterization and pathogenicity evaluation of enterovirus G isolated from diarrheic piglets

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dc.contributor.authorIbrahim, Y.M.
dc.contributor.authorZhang, W.
dc.contributor.authorWang, X.
dc.contributor.authorWerid, G.M.
dc.contributor.authorFu, L.
dc.contributor.authorYu, H.
dc.contributor.authorWang, Y.
dc.contributor.editorHe, B.
dc.date.issued2023
dc.descriptionPublished 13 October 2023
dc.description.abstractFour enterovirus G (EV-G) strains were isolated from diarrheic piglets that were negative for common swine enteric viruses. The spherical enterovirus particles of roughly 30-nm diameter were observed under transmission electron microscopy by using plaque-purified enterovirus. The complete genome sequence analysis revealed that each of four enteroviruses contained a papain-like cysteine protease (PLCP) gene between the 2C and 3A junction regions of the viral genome. This insertion encoded a predicted protease similar to the PLCP of porcine torovirus. The phylogenetic analysis based on complete genome with and without PLCP gene revealed that the four isolated EV-G strains were grouped together with global enterovirus G1-PLCP strains, and more closely related to EV-G/PLCP strains previously detected in China, Japan, and Korea (90.3%–92.2% similarities based on nucleotides). The cell susceptibility test demonstrated that the isolated EV-G could infect and replicate in cell lines from various host species. Furthermore, pathogenicity evaluation showed that the isolated EV-Gs induced mild diarrhea, pyrexia, and reduced body weight in infected piglets. The epidemiological investigation revealed a high prevalence of EV-G in swine herds. Together, our findings demonstrate that the isolated EV-G is pathogenic in piglets and may be advantageous in providing more trustworthy data on the evolution and pathological properties of EV-G.
dc.description.statementofresponsibilityYassein M. Ibrahim, Wenli Zhang, Xinrong Wang, Gebremeskel Mamu Werid, Lizhi Fu, Haidong Yu, Yue Wang
dc.identifier.citationMicrobiology Spectrum, 2023; 11(6)
dc.identifier.doi10.1128/spectrum.02643-23
dc.identifier.issn2165-0497
dc.identifier.issn2165-0497
dc.identifier.orcidWerid, G.M. [0000-0002-3189-1470]
dc.identifier.urihttps://hdl.handle.net/2440/139747
dc.language.isoen
dc.publisherAmerican Society for Microbiology
dc.rightsCopyright © 2023 Ibrahim et al. This is an openaccess article distributed under the terms of the Creative Commons Attribution 4.0 International license.
dc.source.urihttps://doi.org/10.1128/spectrum.02643-23
dc.subjectenterovirus G; characterization; genetic recombination; pathogenicity
dc.titleMolecular characterization and pathogenicity evaluation of enterovirus G isolated from diarrheic piglets
dc.typeJournal article
pubs.publication-statusPublished

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