Studying RNA-protein interactions in vivo by RNA immunoprecipitation
Date
2011
Authors
Selth, L.
Close, T.
Svejstrup, J.
Editors
Tollefsbol, T.
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Book chapter
Citation
Epigenetics Protocols, 2011 / Tollefsbol, T. (ed./s), vol.791, pp.253-264
Statement of Responsibility
Luke A. Selth, Pierre Close and Jesper Q. Svejstrup
Conference Name
Abstract
The crucial roles played by RNA-binding proteins in all aspects of RNA metabolism, particularly in the regulation of transcription, have become increasingly evident. Moreover, other factors that do not directly interact with RNA molecules can nevertheless function proximally to RNA polymerases and have significant effects on gene expression. RNA immunoprecipitation (RIP) is a powerful technique used to detect direct and indirect interactions between individual proteins and specific RNA molecules in vivo. Here, we describe RIP methods for both yeast and mammalian cells.