Studying RNA-protein interactions in vivo by RNA immunoprecipitation

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dc.contributor.authorSelth, L.
dc.contributor.authorClose, T.
dc.contributor.authorSvejstrup, J.
dc.contributor.editorTollefsbol, T.
dc.date.issued2011
dc.description.abstractThe crucial roles played by RNA-binding proteins in all aspects of RNA metabolism, particularly in the regulation of transcription, have become increasingly evident. Moreover, other factors that do not directly interact with RNA molecules can nevertheless function proximally to RNA polymerases and have significant effects on gene expression. RNA immunoprecipitation (RIP) is a powerful technique used to detect direct and indirect interactions between individual proteins and specific RNA molecules in vivo. Here, we describe RIP methods for both yeast and mammalian cells.
dc.description.statementofresponsibilityLuke A. Selth, Pierre Close and Jesper Q. Svejstrup
dc.identifier.citationEpigenetics Protocols, 2011 / Tollefsbol, T. (ed./s), vol.791, pp.253-264
dc.identifier.doi10.1007/978-1-61779-316-5_19
dc.identifier.isbn9781617793158
dc.identifier.orcidSelth, L. [0000-0002-4686-1418]
dc.identifier.urihttp://hdl.handle.net/2440/71701
dc.language.isoen
dc.publisherHumana Press
dc.publisher.placeUnited States
dc.source.urihttps://doi.org/10.1007/978-1-61779-316-5_19
dc.subjectRNA immunoprecipitation
dc.subjectRNA-binding protein
dc.subjecttranscription factor
dc.subjectmRNA
dc.subjectchromatin immunoprecipitation
dc.subjectgene expression
dc.titleStudying RNA-protein interactions in vivo by RNA immunoprecipitation
dc.typeBook chapter
pubs.publication-statusPublished

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