Label Free Assessment of Key Biological Autofluorophores: Material Characteristics and Opportunities for Clinical Applications

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dc.contributor.authorCampbell, J.M.
dc.contributor.authorGosnell, M.
dc.contributor.authorAgha, A.
dc.contributor.authorHandley, S.
dc.contributor.authorKnab, A.
dc.contributor.authorBhargava, A.
dc.contributor.authorGoldys, E.M.
dc.date.issued2024
dc.description.abstractAutofluorophores are endogenous fluorescent compounds that naturally occur in the intra and extracellular spaces of all tissues and organs. Most have vital biological functions - like the metabolic cofactors NAD(P)H and FAD<sup>+</sup>, as well as the structural protein collagen. Others have been considered to be waste products - like lipofuscin and advanced glycation end products - which accumulate with age and are associated with cellular dysfunction. Due to their natural fluorescence these materials have great utility for enabling non-invasive, label-free assays with direct ties to biological function. Numerous technologies, with different advantages and drawbacks, have been applied to their assessment, including fluorescence lifetime imaging microscopy, hyperspectral microscopy, and flow cytometry. Here, we review the applications of label-free autofluorophore assessment for clinical and health-research applications, with specific attention to biomaterials, disease detection, surgical guidance, treatment monitoring and tissue assessment - fields which greatly benefit from non-invasive methodologies capable of continuous, in vivo characterisation. This article is protected by copyright. All rights reserved.
dc.description.statementofresponsibilityJared M. Campbell, Martin Gosnell, Adnan Agha, Shannon Handley, Aline Knab, Ayad G. Anwer, Akanksha Bhargava, and Ewa M. Goldys
dc.identifier.citationAdvanced Materials, 2024; 36(42):e2403761-1-e2403761-28
dc.identifier.doi10.1002/adma.202403761
dc.identifier.issn0935-9648
dc.identifier.issn0935-9648
dc.identifier.orcidCampbell, J.M. [0000-0003-0163-4251]
dc.identifier.urihttps://hdl.handle.net/2440/142854
dc.language.isoen
dc.publisherWiley
dc.relation.granthttp://purl.org/au-research/grants/arc/DP210102960
dc.rights© 2024 The Author(s). Advanced Materials published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
dc.source.urihttp://dx.doi.org/10.1002/adma.202403761
dc.subjectautofluorescence
dc.subjectbiomaterials
dc.subjectdiagnosis
dc.subjectspectral
dc.titleLabel Free Assessment of Key Biological Autofluorophores: Material Characteristics and Opportunities for Clinical Applications
dc.typeJournal article
pubs.publication-statusPublished

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