Strategies for the optimisation of troublesome peptide nucleic acid (PNA) sequences

Date

2025

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Watson, E.E.

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Journal article

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Organic and Biomolecular Chemistry, 2025; 1-18

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Emma E. Watson

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Abstract

Through the use of a pseudo-peptidic backbone, peptide nucleic acids (PNA) mimic the functionality of native nucleic acids while enjoying improved binding affinity and metabolic stability. However, many aspects of the application of PNA to biological and medicinal settings still requires sequence specific optimisation. This review highlights key areas for refinement, including synthesis, tuning of physical properties, cell permeability and analysis, including common strategies for the pracitioner to apply in each area.

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OnlinePubl.

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© The Royal Society of Chemistry 2025

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