Plant CLE peptides from two distinct functional classes synergistically induce division of vascular cells

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dc.contributor.authorWhitford, R.
dc.contributor.authorFernandez, A.
dc.contributor.authorDe Groodt, R.
dc.contributor.authorOrtega, E.
dc.contributor.authorHilson, P.
dc.date.issued2008
dc.description.abstractThe Clavata3 (CLV3)/endosperm surrounding region (CLE) signaling peptides are encoded in large plant gene families. CLV3 and the other A-type CLE peptides promote cell differentiation in root and shoot apical meristems, whereas the B-type peptides (CLE41–CLE44) do not. Instead, CLE41 inhibits the differentiation of Zinnia elegans tracheary elements. To test whether CLE genes might code for antagonistic or synergistic functions, peptides from both types were combined through overexpression within or application onto Arabidopsis thaliana seedlings. The CLE41 peptide (CLE41p) promoted proliferation of vascular cells, although delaying differentiation into phloem and xylem cell lineages. Application of CLE41p or overexpression of CLE41 did not suppress the terminal differentiation of the root and shoot apices triggered by A-type CLE peptides. However, in combination, A-type peptides enhanced all of the phenotypes associated with CLE41 gain-of-function, leading to massive proliferation of vascular cells. This proliferation relied on auxin signaling because it was enhanced by exogenous application of a synthetic auxin, decreased by an auxin polar transport inhibitor, and abolished by a mutation in the Monopteros auxin response factor. These findings highlight that vascular patterning is a process controlled in time and space by different CLE peptides in conjunction with hormonal signaling.
dc.description.statementofresponsibilityRyan Whitford, Ana Fernandez, Ruth De Groodt, Esther Ortega, and Pierre Hilson
dc.identifier.citationProceedings of the National Academy of Sciences of USA, 2008; 105(47):18625-18630
dc.identifier.doi10.1073/pnas.0809395105
dc.identifier.issn0027-8424
dc.identifier.issn1091-6490
dc.identifier.orcidWhitford, R. [0000-0003-2263-7558]
dc.identifier.urihttp://hdl.handle.net/2440/54718
dc.language.isoen
dc.publisherNatl Acad Sciences
dc.source.urihttps://doi.org/10.1073/pnas.0809395105
dc.subjectPeptides
dc.subjectPlant Proteins
dc.subjectCell Division
dc.subjectCell Lineage
dc.subjectPlant Physiological Phenomena
dc.subjectPlant Cells
dc.subjectPlant Development
dc.titlePlant CLE peptides from two distinct functional classes synergistically induce division of vascular cells
dc.typeJournal article
pubs.publication-statusPublished

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