Multifunctional Hydroxyapatite Coated with Gallium Liquid Metal-Based Silver Nanoparticles for Infection Prevention and Bone Regeneration

Simple item page
dc.contributor.authorNguyen, N.H.
dc.contributor.authorZhang, P.
dc.contributor.authorKadavan, F.S.P.
dc.contributor.authorXu, Z.
dc.contributor.authorNguyen, T.T.
dc.contributor.authorLi, W.
dc.contributor.authorNguyen, M.T.
dc.contributor.authorNguyen, C.K.
dc.contributor.authorPham, D.Q.
dc.contributor.authorPham, T.G.T.
dc.contributor.authorHayles, A.
dc.contributor.authorAlemie, M.N.
dc.contributor.authorVongsvivut, J.
dc.contributor.authorChan, V.
dc.contributor.authorKidd, S.P.
dc.contributor.authorZhao, Y.
dc.contributor.authorVasilev, K.
dc.contributor.authorTruong, V.K.
dc.date.issued2025
dc.descriptionOnlinePubl
dc.description.abstractImplant-associated infections and inflammation during pre- and post-operative procedures remain significant challenges that reduce implant longevity. In this study, the uniques bioactive coatings derived from silver-gallium liquid metal particles (Ag-GaNPs) deposited uniformly over HAp scaffold (HAp-Ag-GaNPs) are developed. The HAp-Ag-GaNPs exhibit strong antimicrobial activities against Gram-negative Pseudomonas aeruginosa, Gram-positive Staphylococcus aureus (S. aureus), drug-resistant bacteria such as methicillin-resistant S. aureus, and persistent bacteria like small colony variant of S. aureus. The antibacterial mechanisms of HAp-Ag-GaNPs are multifaced, including reactive oxygen species within cells, leading to damage and leakage of cytosolic contents and reduced ATP levels. The synchrotron macro attenuated total reflectance – Fourier transform infrared microspectroscopy is utilized to understand the influence of HAp-Ag-GaNPs on lipids, proteins, and nucleic acids of pathogenic bacteria. Proteomic analysis reveals that HAp-Ag-GaNPs disrupt critical bacterial processes, including DNA replication, RNA transcription, protein synthesis, and energy metabolism, alongside inducing oxidative stress and membrane damage in bacteria. In addition, in vivo studies demonstrate reduced bacterial colonization and enhanced tissue integration at implant sites treated with HAp-Ag-GaNPs, further supporting their dual functionality. The findings highlight the potential of HAp-Ag-GaNPs as a next-generation biomaterial with dual antibacterial and osteogenic properties for clinical applications in orthopedic implants.
dc.description.statementofresponsibilityNgoc Huu Nguyen, Pengfei Zhang, Fathima Shana Pattar Kadavan, Zhaoning Xu, Tien Thanh Nguyen, Wenshao Li, Manh Tuong Nguyen, Chung Kim Nguyen, Duy Quang Pham, Thi Giang Tuyet Pham, Andrew Hayles, Markos Negash Alemie, Jitraporn Vongsvivut, Vincent Chan, Stephen Peter Kidd, Yunpeng Zhao, Krasimir Vasilev, and Vi Khanh Truong
dc.identifier.citationAdvanced Functional Materials, 2025; 2423496 -1-2423496 -22
dc.identifier.doi10.1002/adfm.202423496
dc.identifier.issn1616-301X
dc.identifier.issn1616-3028
dc.identifier.orcidKidd, S.P. [0000-0002-2118-1651]
dc.identifier.urihttps://hdl.handle.net/2440/147041
dc.language.isoen
dc.publisherWiley
dc.relation.grantRC2-2018-022
dc.relation.grantFSU-2025-004
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/GNT1194466
dc.rights© 2025 The Author(s). Advanced Functional Materials published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
dc.source.urihttps://doi.org/10.1002/adfm.202423496
dc.subjectantimicrobial; biocompatibility; hydroxyapatite; liquid metal; silver-gallium; bone regeneration
dc.titleMultifunctional Hydroxyapatite Coated with Gallium Liquid Metal-Based Silver Nanoparticles for Infection Prevention and Bone Regeneration
dc.typeJournal article
pubs.publication-statusPublished online

Files

Collections

Research Outputs