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Isolation of circulating fetal trophoblasts using inertial microfluidics for noninvasive prenatal testing

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dc.contributor.authorWinter, M.
dc.contributor.authorHardy, T.
dc.contributor.authorRezaei, M.
dc.contributor.authorNguyen, V.
dc.contributor.authorZander, D.
dc.contributor.authorEbrahimi Warkiani, M.
dc.contributor.authorThierry, B.
dc.date.issued2018
dc.description.abstractWhile noninvasive prenatal testing based on cell‐free fetal DNA has recently revolutionized the field of aneuploidy screening in pregnancy, it remains limited to aneuploidy and microdeletion screening, and is unable to reliably detect single gene disorders. A number of recent studies have demonstrated the potential of circulating trophoblastic cells in providing cell‐based noninvasive diagnosis with sequencing or array‐based assays. However, considering the extreme rarity of these cells in blood, efficient, high‐throughput, and clinically applicable enrichment technologies are yet to be developed. This study demonstrates for the first time the utility of inertial microfluidics for efficient isolation of trophoblastic cells from maternal peripheral blood. Under optimal operating conditions, high‐recovery yields (79%) are obtained using a trophoblastic cell‐line, which is subsequently confirmed with analysis of maternal blood. Feasibility of obtaining a diagnosis from cells isolated from a maternal sample is demonstrated in a case of confirmed fetal trisomy 21 in which six fetal cells are found in a 7 mL blood sample using fluorescence in situ hybridization. Finally, it is demonstrated that trophoblastic cells isolated using inertial microfluidics could be picked and subjected to a clinically validated sequencing assay, paving the way for further validation of this technology and larger clinical studies.
dc.description.statementofresponsibilityMarnie Winter, Tristan Hardy, Meysam Rezaei, Vy Nguyen, Deirdre Zander-Fox, Majid Ebrahimi Warkiani and Benjamin Thierry
dc.identifier.citationAdvanced Materials Technologies, 2018; 3(7):1800066-1-1800066-10
dc.identifier.doi10.1002/admt.201800066
dc.identifier.issn2365-709X
dc.identifier.issn2365-709X
dc.identifier.orcidWinter, M. [0000-0002-7499-789X]
dc.identifier.orcidHardy, T. [0000-0002-5878-0340]
dc.identifier.urihttp://hdl.handle.net/2440/120845
dc.language.isoen
dc.publisherWiley
dc.relation.grantARC
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1045841
dc.rights© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
dc.source.urihttps://doi.org/10.1002/admt.201800066
dc.subjectImaging flow cytometry; inertial microfluidics; noninvasive prenatal testing; trophoblastic cell isolation
dc.titleIsolation of circulating fetal trophoblasts using inertial microfluidics for noninvasive prenatal testing
dc.typeJournal article
pubs.publication-statusPublished

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