InP/ZnS nanocrystals as fluorescent probes for the detection of ATP
Files
(Published version)
Date
2014
Authors
Massadeh, S.
Nann, T.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Nanomaterials and Nanotechnology, 2014; 4(1):1-8
Statement of Responsibility
Conference Name
DOI
Abstract
This article reports on a study on fluorescence adenosine triphosphate (ATP) detection by InP/ZnS quantum dots (QDs). We present a spectroscopic analysis displaying the effect of enzymatic reactions of glucose oxidase (GOX) and hexokinase (HEX) on the InP/ZnS quantum dots at physiological pH. The InP/ZnS quantum dots act as glucose sensors in the presence of GOX, Glu and ATP, and their luminescence quenches during the release of hydrogen peroxide from the reaction. However, in the presence of adenosine 5' triphosphate, glucose, and HEX, a significant photobrightening of the InP/ZnS QDs is recorded. This is dependent on the concentration of ATP in the sample. The relationship between the ATP and the emission intensity of InP/ZnS nanocrystals is linear. The present results are the first to report the effect of different by-products released by these enzymatic reactions on the fluorescence of the InP/ZnS QDs.
School/Discipline
Dissertation Note
Provenance
Description
Access Status
Rights
Copyright 2014 The Author(s). Licensee InTech. This is an open access article distributed under the terms of the CreativeCommons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use,distribution, and reproduction in any medium, provided the original work is properly cited. (http://creativecommons.org/licenses/by/3.0)