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InP/ZnS nanocrystals as fluorescent probes for the detection of ATP

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dc.contributor.authorMassadeh, S.
dc.contributor.authorNann, T.
dc.date.issued2014
dc.description.abstractThis article reports on a study on fluorescence adenosine triphosphate (ATP) detection by InP/ZnS quantum dots (QDs). We present a spectroscopic analysis displaying the effect of enzymatic reactions of glucose oxidase (GOX) and hexokinase (HEX) on the InP/ZnS quantum dots at physiological pH. The InP/ZnS quantum dots act as glucose sensors in the presence of GOX, Glu and ATP, and their luminescence quenches during the release of hydrogen peroxide from the reaction. However, in the presence of adenosine 5' triphosphate, glucose, and HEX, a significant photobrightening of the InP/ZnS QDs is recorded. This is dependent on the concentration of ATP in the sample. The relationship between the ATP and the emission intensity of InP/ZnS nanocrystals is linear. The present results are the first to report the effect of different by-products released by these enzymatic reactions on the fluorescence of the InP/ZnS QDs.
dc.identifier.citationNanomaterials and Nanotechnology, 2014; 4(1):1-8
dc.identifier.doi10.5772/58523
dc.identifier.issn1847-9804
dc.identifier.issn1847-9804
dc.identifier.urihttps://hdl.handle.net/1959.8/159770
dc.language.isoen
dc.publisherInTech
dc.relation.fundingEngineering and Physical Sciences Research Council (EPSRC)
dc.rightsCopyright 2014 The Author(s). Licensee InTech. This is an open access article distributed under the terms of the CreativeCommons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use,distribution, and reproduction in any medium, provided the original work is properly cited. (http://creativecommons.org/licenses/by/3.0)
dc.source.urihttps://doi.org/10.5772/58523
dc.subjectadenosine triphosphate
dc.subjectnanocrystals
dc.subjectnanosensora
dc.subjectphotoenhancement
dc.subjectquantum dots
dc.titleInP/ZnS nanocrystals as fluorescent probes for the detection of ATP
dc.typeJournal article
pubs.publication-statusPublished
ror.fileinfo12143330950001831 13143359830001831 9915914034301831_53137159100001831.pdf
ror.mmsid9915914034301831

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