Variable expressivity of the tumour suppressor protein TRP53 in cryopreserved human blastocysts

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dc.contributor.authorChandrakanthan, V.
dc.contributor.authorChami, O.
dc.contributor.authorStojanov, T.
dc.contributor.authorO'Neill, C.
dc.date.issued2007
dc.description.abstractIn a mouse model, in vitro fertilization or extended embryo culture leads to the increased expression of TRP53 in susceptible embryos. Ablation of the TRP53 gene improved embryo viability indicating that increased expression of TRP53 is a cause of the reduction of embryo viability resulting from in vitro fertilization or embryo culture. This study investigates the status of TRP53 expression in human embryos produced by intracytoplasmic sperm injection. Following fertilization, embryos were cultured for 96 h and then cryopreserved. Immediately upon thawing they were fixed in formaldehyde and subjected to immunostaining for TRP53. Staining was visualized by confocal microscopy. Negative controls were incubated with isotype control immunoglobulin and showed negligible staining. All embryos showed TRP53 staining above negative controls. TRP53 staining was heterogenous within and between embryos. An embryo that showed retarded development showed high levels of TRP53 expression. A blastocyst that had a collapsed blastocoel also showed high levels of TRP53 compared to morphologically normal blastocysts. Most TRP53 staining was in the region of the nucleus. Morphologically normal blastocysts tended to show little nuclear accumulation of stain. However, some cells within these embryos had high levels of nuclear TRP53 expression. The results show that embryos have varying sensitivity to the stresses of production and culture in vitro, and this resulted in variable expressivity of TRP53.
dc.description.statementofresponsibilityVashe Chandrakanthan, Omar Chami, Tomas Stojanov, and Chris O'Neill
dc.identifier.citationReproductive Biology and Endocrinology, 2007; 5(1):39-1-39-6
dc.identifier.doi10.1186/1477-7827-5-39
dc.identifier.issn1477-7827
dc.identifier.issn1477-7827
dc.identifier.orcidChandrakanthan, V. [0000-0002-4314-5029]
dc.identifier.urihttps://hdl.handle.net/2440/145938
dc.language.isoen
dc.publisherBMC
dc.relation.grantNHMRC
dc.rights© 2007 Chandrakanthan et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.source.urihttps://doi.org/10.1186/1477-7827-5-39
dc.subjectHuman Embryo; Embryo Culture; Intracytoplasmic Sperm Injection; Developmental Potential; Embryo Viability
dc.subject.meshBlastocyst
dc.subject.meshHumans
dc.subject.meshCryopreservation
dc.subject.meshEmbryo Culture Techniques
dc.subject.meshFemale
dc.subject.meshMale
dc.subject.meshTumor Suppressor Protein p53
dc.titleVariable expressivity of the tumour suppressor protein TRP53 in cryopreserved human blastocysts
dc.typeJournal article
pubs.publication-statusPublished

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