Efficient regio- and stereo-selective C-H bond hydroxylation of steroids using an engineered heme-thiolate peroxygenase biocatalyst

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dc.contributor.authorAkter, J.
dc.contributor.authorHayball, E.F.
dc.contributor.authorBell, S.G.
dc.date.issued2023
dc.description.abstractA crucial reaction in chemical synthesis is the activation of unactivated carbon–hydrogen bonds in complex molecules. We demonstrate the regio- and stereo-selective hydroxlation of the steroids progesterone and androstenedione using the peroxygenase activity of an engineered bacterial cytochrome P450 enzyme, CYP154C8. By replacing a single amino acid of the I-helix we change this monooxygenase enzyme into a peroxygenase, enabling the efficient and selective biocatalytic formation of the 16α-hydroxy steroid metabolite.
dc.description.statementofresponsibilityJinia Akter, Eva F. Hayball and Stephen G. Bell
dc.identifier.citationCatalysis Science & Technology, 2023; 13(22):6355-6359
dc.identifier.doi10.1039/d3cy01223a
dc.identifier.issn2044-4753
dc.identifier.issn2044-4761
dc.identifier.orcidAkter, J. [0000-0003-1798-474X]
dc.identifier.orcidHayball, E.F. [0000-0001-9026-8630]
dc.identifier.orcidBell, S.G. [0000-0002-7457-9727]
dc.identifier.urihttps://hdl.handle.net/2440/141709
dc.language.isoen
dc.publisherRoyal Society of Chemistry (RSC)
dc.relation.granthttp://purl.org/au-research/grants/arc/DP200102411
dc.rightsThis journal is © The Royal Society of Chemistry 2023
dc.source.urihttps://doi.org/10.1039/d3cy01223a
dc.subjectcarbon-hydrogen; molecules
dc.titleEfficient regio- and stereo-selective C-H bond hydroxylation of steroids using an engineered heme-thiolate peroxygenase biocatalyst
dc.typeJournal article
pubs.publication-statusPublished

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