Transcriptomic Response in the Spleen after Whole-Body Low-Dose X-Ray Irradiation.
Date
2021
Authors
Puukila, S.
Tharmalingam, S.
Al-Khayyat, W.
Peterson, J.
Hooker, A.M.
Muise, S.
Boreham, D.R.
Dixon, D.-L.
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Journal article
Citation
Radiation Research, 2021; 196(1):66-73
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S. Puukila, S. Tharmalingam, W. Al-Khayyat, J. Peterson, A. M. Hooker, S. Muise, D. R. Boreham, D-L. Dixon
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Abstract
As the use of medical radiation procedures continues to rise, it is imperative to further our understanding of the effects of this exposure. The spleen is not known as a particularly radiosensitive organ, although its tolerance to radiation is not well understood. Low-dose radiation exposure has been implicated in beneficial responses, particularly in cell death and DNA damage repair. In this study, adult male rats received 2, 20, 200 mGy or 4 Gy whole-body X-ray irradiation and the transcriptional response in the spleen was analyzed at 0.5, 4 and 24 h postirradiation. We analyzed expression of genes involved in apoptosis, cell cycle progression and DNA damage repair. As expected, 4 Gy irradiated animals demonstrated elevated expression of genes related to apoptosis at 0.5, 4 and 24 h postirradiation in the spleen. These animals also showed upregulation of DNA damage repair genes at 24 h postirradiation. Interestingly, the spleens of 20 mGy irradiated animals showed reduced apoptosis and cell cycle arrest compared to the spleens of sham-irradiated animals. These results further reveal that the cellular response in the spleen to whole-body irradiation differs between low- and high-dose irradiation.
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© 2021 by Radiation Research Society. All rights of reproduction in any form reserved.