Transcriptomic Response in the Spleen after Whole-Body Low-Dose X-Ray Irradiation.

Simple item page
dc.contributor.authorPuukila, S.
dc.contributor.authorTharmalingam, S.
dc.contributor.authorAl-Khayyat, W.
dc.contributor.authorPeterson, J.
dc.contributor.authorHooker, A.M.
dc.contributor.authorMuise, S.
dc.contributor.authorBoreham, D.R.
dc.contributor.authorDixon, D.-L.
dc.date.issued2021
dc.description.abstractAs the use of medical radiation procedures continues to rise, it is imperative to further our understanding of the effects of this exposure. The spleen is not known as a particularly radiosensitive organ, although its tolerance to radiation is not well understood. Low-dose radiation exposure has been implicated in beneficial responses, particularly in cell death and DNA damage repair. In this study, adult male rats received 2, 20, 200 mGy or 4 Gy whole-body X-ray irradiation and the transcriptional response in the spleen was analyzed at 0.5, 4 and 24 h postirradiation. We analyzed expression of genes involved in apoptosis, cell cycle progression and DNA damage repair. As expected, 4 Gy irradiated animals demonstrated elevated expression of genes related to apoptosis at 0.5, 4 and 24 h postirradiation in the spleen. These animals also showed upregulation of DNA damage repair genes at 24 h postirradiation. Interestingly, the spleens of 20 mGy irradiated animals showed reduced apoptosis and cell cycle arrest compared to the spleens of sham-irradiated animals. These results further reveal that the cellular response in the spleen to whole-body irradiation differs between low- and high-dose irradiation.
dc.description.statementofresponsibilityS. Puukila, S. Tharmalingam, W. Al-Khayyat, J. Peterson, A. M. Hooker, S. Muise, D. R. Boreham, D-L. Dixon
dc.identifier.citationRadiation Research, 2021; 196(1):66-73
dc.identifier.doi10.1667/rade-20-00267.1
dc.identifier.issn0033-7587
dc.identifier.issn1938-5404
dc.identifier.orcidHooker, A.M. [0000-0003-2324-4748]
dc.identifier.orcidDixon, D.-L. [0000-0001-6459-1856]
dc.identifier.urihttps://hdl.handle.net/2440/146547
dc.language.isoen
dc.publisherRadiation Research Society
dc.rights© 2021 by Radiation Research Society. All rights of reproduction in any form reserved.
dc.source.urihttps://doi.org/10.1667/rade-20-00267.1
dc.subjectMedical radiation procedures; Effects; Side effects; Medical; Spleen; Cell cycle
dc.subject.meshSpleen
dc.subject.meshAnimals
dc.subject.meshRats
dc.subject.meshRats, Sprague-Dawley
dc.subject.meshDNA Damage
dc.subject.meshWhole-Body Irradiation
dc.subject.meshCell Cycle
dc.subject.meshApoptosis
dc.subject.meshDNA Repair
dc.subject.meshDose-Response Relationship, Drug
dc.subject.meshGenes, cdc
dc.subject.meshX-Rays
dc.subject.meshMale
dc.subject.meshTranscriptome
dc.titleTranscriptomic Response in the Spleen after Whole-Body Low-Dose X-Ray Irradiation.
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Research Outputs