Assessing Seminal Fluid Interaction with Female Reproductive Tract Tissues: In Vivo and In Vitro Models for Mouse
Date
2025
Authors
Chan, H.Y.
Schjenken, J.E.
Sharkey, D.J.
Robertson, S.A.
Editors
Álvarez-Rodríguez, M.
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Book chapter
Citation
Spermatology: Methods and Protocols, 2025 / Álvarez-Rodríguez, M. (ed./s), vol.2897, Ch.44, pp.649-662
Statement of Responsibility
Hon Yeung Chan, John E. Schjenken, David J. Sharkey, and Sarah A. Robertson
Conference Name
Abstract
After intromission of seminal fluid at mating in mammals, the seminal fluid interacts with the lining of the female reproductive tract and elicits a female immune response. This response is triggered by signals in seminal fluid (carried by both the sperm and seminal plasma components) that bind receptors on female tract epithelial cells, causing a change in their transcriptional program to upregulate expression of pro-inflammatory cytokines and chemokines. The ensuing leukocyte recruitment and immune activation facilitates conception, embryo development, and progression of pregnancy. In this chapter, we report methods for evaluating effects of seminal fluid and its components (seminal plasma and sperm) on the female reproductive tract immune response in mice.
School/Discipline
Dissertation Note
Provenance
Description
Series Editor John M. Walker, Print ISSN 1064-3745 ; Electronic ISSN 1940-6029.
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Rights
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025