The prevalence of controls in phyllosphere microbiome research: a methodological review
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2024
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Welsh, B.L.
Eisenhofer, R.
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New Phytologist, 2024; 242(1):23-29
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Brady L. Welsh, Raphael Eisenhofer
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DNA contamination can critically confound microbiome studies. Here, we take a systematic approach to review the current literature and investigate the prevalence of contamination controls in phyllosphere microbiome research over the past decade. By utilising systematic review principles for this review,wewere able to conduct a thorough investigation, screening 450 articles from three databases for eligibility and extracting data in a controlled and methodical manner. Worryingly, we observed a surprisingly low usage of both positive and negative contamination controls in phyllosphere research. As a result, we propose a set of minimum standards to combat the effects of contamination in future phyllosphere research.
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© 2024 The Authors; © 2024 New Phytologist Foundation. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.