The prevalence of controls in phyllosphere microbiome research: a methodological review

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dc.contributor.authorWelsh, B.L.
dc.contributor.authorEisenhofer, R.
dc.date.issued2024
dc.description.abstractDNA contamination can critically confound microbiome studies. Here, we take a systematic approach to review the current literature and investigate the prevalence of contamination controls in phyllosphere microbiome research over the past decade. By utilising systematic review principles for this review,wewere able to conduct a thorough investigation, screening 450 articles from three databases for eligibility and extracting data in a controlled and methodical manner. Worryingly, we observed a surprisingly low usage of both positive and negative contamination controls in phyllosphere research. As a result, we propose a set of minimum standards to combat the effects of contamination in future phyllosphere research.
dc.description.statementofresponsibilityBrady L. Welsh, Raphael Eisenhofer
dc.identifier.citationNew Phytologist, 2024; 242(1):23-29
dc.identifier.doi10.1111/nph.19573
dc.identifier.issn0028-646X
dc.identifier.issn1469-8137
dc.identifier.orcidRoff, A.J. [0000-0003-2560-7629]
dc.identifier.orcidTai, A. [0000-0001-9240-5416]
dc.identifier.orcidMuhlhausler, B.S. [0000-0002-9021-6790]
dc.identifier.orcidClifton, V.L. [0000-0002-4892-6748]
dc.identifier.orcidGatford, K.L. [0000-0002-2823-3004]
dc.identifier.urihttps://hdl.handle.net/2440/146589
dc.language.isoen
dc.publisherWiley
dc.rights© 2024 The Authors; © 2024 New Phytologist Foundation. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
dc.source.urihttp://dx.doi.org/10.1111/nph.19573
dc.subjectcontamination; controls; epiphytic; microbiome; microbiota; phyllosphere; plants; standards.
dc.subject.meshPrevalence
dc.subject.meshSystematic Reviews as Topic
dc.subject.meshMicrobiota
dc.titleThe prevalence of controls in phyllosphere microbiome research: a methodological review
dc.typeJournal article
pubs.publication-statusPublished

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