Heterogeneous immunoreactivity of axonal spheroids in focal symmetrical encephalomalacia produced by Clostridium perfringens type D epsilon toxin in sheep

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dc.contributor.authorManavis, J.
dc.contributor.authorBlumbergs, P.
dc.contributor.authorJerrett, I.
dc.contributor.authorHanshaw, D.
dc.contributor.authorUzal, F.
dc.contributor.authorFinnie, J.
dc.date.issued2021
dc.description.abstractSince axonal injury (AI) is an important component of many veterinary neurologic disorders, we assessed the relative ability of a panel of antibodies (amyloid precursor protein, 3 subunits of neurofilament protein, protein gene product 9.5, ubiquitin, and synaptophysin) to detect axonal swellings or spheroids. Abundant axonal spheroids found in necrotic internal capsule foci produced in 4 sheep by chronic Clostridium perfringens type D epsilon neurotoxicity provided a model system in which to evaluate this important diagnostic tool. There was heterogeneous labeling of subsets of spheroids by the respective antibodies, suggesting that, in order to detect the complete spectrum of AI in diagnostic cases, a range of antibodies should be used, not only when spheroids are plentiful but also when they are few in number or incompletely developed. The application of insufficient markers in the latter cases can potentially lead to the contribution of AI to lesion pathogenesis being underappreciated.
dc.description.statementofresponsibilityJim Manavis, Peter Blumbergs, Ian Jerrett, Daren Hanshaw, Francisco Uzal, and John Finnie
dc.identifier.citationVeterinary pathology, 2021; 59(2):328-332
dc.identifier.doi10.1177/03009858211057222
dc.identifier.issn0300-9858
dc.identifier.issn0300-9858
dc.identifier.orcidManavis, J. [0000-0001-7381-7781] [0000-0003-1268-561X]
dc.identifier.orcidFinnie, J. [0000-0003-2277-1693]
dc.identifier.urihttps://hdl.handle.net/2440/146008
dc.language.isoen
dc.publisherSAGE Publications
dc.rights© The Author(s) 2021
dc.source.urihttps://doi.org/10.1177/03009858211057222
dc.subjectaxonal spheroids; brain; clostridial epsilon toxin; immunohistochemistry; sheep
dc.subject.meshAnimals
dc.subject.meshSheep
dc.subject.meshClostridium perfringens
dc.subject.meshEncephalomalacia
dc.subject.meshSheep Diseases
dc.subject.meshNecrosis
dc.titleHeterogeneous immunoreactivity of axonal spheroids in focal symmetrical encephalomalacia produced by Clostridium perfringens type D epsilon toxin in sheep
dc.typeJournal article
pubs.publication-statusPublished

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